The simultaneous isolation of outer hair cells and Deiters 'cells from the cochlea of the guinea pig 豚鼠耳蜗外毛细胞和Deiters细胞的同时分离法
Objective: To investigate the method of isolating partial outer supporting cells ( Deiters 'cells, Hensen cells and Outer pillar cells) from guinea-pig cochlea and to try to establish the morphological criteria for these outer supporting cells viability. 目的:探讨豚鼠耳蜗部分外支持细胞(Deiter细胞、Hensen细胞和外柱细胞)的分离方法,并建立细胞活性的鉴别标准。
Objective: To investigate the simultaneous method of isolating outer hair cells and Deiters 'cells from the organ of Corti of the guinea pig cochlea. 目的:探讨豚鼠耳蜗外毛细胞(OHC)和Deiters细胞(DC)同时分离的方法。
Result: CGRP immunoreactivity was located in SGN, nerve fibers at osseous spiral lamina, stria vascularis, IHC, adjacent area of OHC and Deiters 'cells. 结果:CGRP阳性反应物分布于螺旋神经元,骨螺旋板神经孔,内毛细胞、外毛细胞与外支持细胞交界区,螺旋韧带血管纹。